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粪便DNA提取试剂盒
发布时间:2015-11-20
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 快速高效提取粪便DNA,可裂解任何粪便样品。可直接用于PCR等其下游实验,配合FastPrep仪器使用无需研磨样品。

产品名称:粪便DNA试剂盒FastDNA Spin Kit For Feces(MP) 

产品规格:1 kit 50preps

产品货号:116570200

储存温度:15℃-30

产品描述,如下:

The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.

Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.

产品应用,如下:

The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.

产品属性,如下:

Product Type

Kits & Assays

Components

Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each

Instrument Compatibility

FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1

Sample Type

Soil; Feces; Environmental Water; Wastewater; Sludge

Sample Volume

up to 500 mg per prep

Tube Compatibility

2 mL

Matrix Type

E

Molecule Enriched

DNA

 

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