SYBR^® Select Master Mix：SYBR^® 源厂的全新一代荧光定量 PCR预混液

— 前所未有的 性能， 惊心动魄的 优惠。 

一．产品介绍 
全新一代荧光定量PCR预混液，SYBR Select Master Mix： 

·        特异性更高—引物二聚体和非特异性扩增降至最低

·        极高的灵敏度—可实现单拷贝检测

·        GreenER™染料—饱和染料荧光信号更亮

·        残余污染控制—热不稳定性UDG酶，清除残余DNA污染

·        广泛的兼容性—适用于绝大多数实时荧光定量PCR仪

二、产品性能：

1. 线性范围更宽广，扩增曲线更饱满
图1  左图SYBR® Select Master  Mix在不同范围的起始浓度cDNA，均可获得极佳的扩增曲线和分辨能力。采用人cDNA扩增PGK1的扩增曲线和稀释曲线。至多可检测100 ng的起始DNA，无PCR抑制性，不降低PCR效率。右图竞争对手T的SYBR预混液，DNA起始浓度低时，扩增曲线重复性极差，分辨能力低。DNA起始浓度高时会抑制PCR扩增。
2. 极佳的特异性
图2  SYBR® Select Master  Mix极佳的特异性已经过44种Assay测试，每个Assay的熔解曲线均获得了单独的Tm峰。代表性的8种Assay熔解曲线如图所示。
3. 单拷贝的灵敏检测
图3  采用数字qPCR进行单拷贝检测。将基因组DNA稀释至0.5拷贝/反应孔，确保绝大多数孔中含有1或0拷贝DNA。检测单拷贝RNA酶P基因。上图，使用SYBR® Select Master  Mix，扩增后获得的Ct值的正态分布图。所有扩增产物的熔解曲线都形成单峰 ，无非特异性扩增，真正实现单拷贝检测。下图，竞争对手B的SYBR预混液，Ct值随机分布，熔解曲线出现多峰，存在大量非特异性的扩增，无法实现单拷贝检测。
注：SYBR® SelectMaster Mix不仅能在AppliedBiosystems的仪器上运行优良，而且在其他品牌的仪器上也同样可获得完好的结果，其配方经过优化并通过实验证明。

描述

SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility

Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction and can reliably detect a single copy of genomic DNA.

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR® Select Master Mix is used in standard cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

内容和储存

The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 5 mL tube, sufficient for 500, 20 µL reactions (10 µL Master Mix per reaction) . Store at 2 - 8 °C.

详细说明

常用规格