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  • 产品名称:DIG High Prime Lab/Detection K
  • 产品货号:11745832910
  • 产品规格:12LR;24DR
  • 产品品牌:ROCHE
  • 供应商:Roche
  • 产品产地: 产品价格:¥询价
  • 产品说明书:
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  • 详细信息:
  • DIG High Prime DNA Labeling and Detection Starter Kit 1Kit for color detection with NBT/BCIP

    Roche公司现为用户提供一种新的DIG标记及检测试盒DIG High Prime DNA Labeling and Detection Kit I。与原有的DIG DNA Labeling and Detection kit(Cat.No.1093657)比较,本产品有以下特点:

    能对10×10cm印迹膜作12个标记反应及24个显色检测反应,是DIG DNA Labeling and Detection Kit(Cat.No.1093657)份量的一半,所以特别适合初用者及需处理较少量样品的用户使用。
    试剂盒采用的是DIG高效标记试剂(Cat.No.1585606),其标记效率是标准DIG随机引物标记法的3-10倍,而且预混了标记需要的所有试剂,即开即用,令操作更简单和方便。
    试剂盒中还包括了DIG定量/对照试纸条,令您能在30分钟内只用小量试剂便可估计DIG标记探针的浓度,方便您取得理想的信噪比。
    用于显色的NBT/BCIP底物为预混的浓缩度,而封闭液则以10倍浓度形式供应,简化了操作程序。

    产品号

    产品规格

    厂商

    价格

    1745832

    11745832910

    Kit1 kit (12 labeling reactions and 24 detection reactions)

    Roche

    6016

     

     

    Application

    DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent. 

    Benefits

    The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled. 

    Product Description

    Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA. 

    Contents

    1.       DIG-High Prime, 5x conc., 50 µl

    2.       DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)

    3.       DNA Dilution Buffer, 3 x 1 ml

    4.       Anti-digoxigenin-AP Conjugate, 100 µl

    5.       NBT/BCIP Stock Solution, conc., 6 x 1 ml

    6.       Blocking Solution, 10x conc., 4 x 100 ml

    DIG Easy Hyb Granules, 4 x 100 ml 

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